The N‐terminal domain of SV40 large T antigen represses the HER2/neu‐mediated transformation and metastatic potential in breast cancers

FEBS Letters

Wang, Shan-Shue³  Kao, Ming-Ching¹  Chuang, Tzu-Chao¹  Lin, Yen-Shing²  Yu, Yuh-Hua² 

[1] Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC;Department of Biochemistry, National Defense Medical Center, Neihu, P.O. Box 90048-501, Taipei 114, Taiwan, ROC;Department of Research and Development, Adpharma Inc., Tainan, Taiwan, ROC

关键词: HER2/neu;    p185;    Metastasis;    Simian virus 40 large T antigen;   

DOI  :  10.1016/S0014-5793(01)03277-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

HER2/neu is known to be overexpressed in approximately 40% of human breast and ovarian cancers and it is associated with increased metastasis and poor prognosis. We have shown previously that the N-terminal domain of simian virus 40 large T antigen (LT425) can act as a transforming suppressor of the HER2/neu oncogene in human ovarian cancer. In the present study, we demonstrate that LT425 can also repress the transforming properties of HER2/neu-overexpressing human breast cancer cells. In addition, the results of a chemotaxis assay and an in vitro chemoinvasion assay further suggest that LT425 can also suppress the metastatic potential of the HER2/neu-transformed breast cancer cells. Taken together, these data clearly suggest that the inhibition of the expression of p185^HER2/neu tyrosine kinase by LT425 is capable of suppressing the HER2/neu-mediated transformation and metastatic potential in breast cancers.

【 授权许可】

Unknown