Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts

Cell Transplantation

Article

Michinori Kohara¹  Michiki Narushima²  Nalú Navarro-Alvarez²  Noriaki Tanaka²  Atsushi Miki²  Alejandro Soto-Gutierrez²  Kimiaki Tanaka²  Jorge David Rivas-Carrillo²  Naoya Kobayashi²  Yong Chen²  Shinichi Matsumoto³  Hirofumi Noguchi³  Teru Okitsu³  Yuichi Takeno⁴  Eiji Kobayashi⁴  Jonathan R. T. Lakey⁵ 

[1] Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, Tokyo 113-8613 Japan;Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan;Department of Transplant Surgery, Kyoto University Hospital, 54 Seigoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan;Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan;Human Pancreatic Islet Transplant Program, University of Alberta, Alberta T2N 4N1, Canada;

关键词: Islets;    Simian virus 40 large T antigen;    Coculture;   

DOI  :  10.3727/000000006783981882

 received in 2005-08-27, accepted in 2005-09-30,  发布年份 2006

来源: Sage Journals

【 摘 要 】

Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.

【 授权许可】

Unknown   
© 2006 Cognizant Comm. Corp.