Cell Transplantation | |
Maintenance of Mouse, Rat, and Pig Pancreatic Islet Functions by Coculture with Human Islet-Derived Fibroblasts | |
Article | |
Michinori Kohara1  Michiki Narushima2  Nalú Navarro-Alvarez2  Noriaki Tanaka2  Atsushi Miki2  Alejandro Soto-Gutierrez2  Kimiaki Tanaka2  Jorge David Rivas-Carrillo2  Naoya Kobayashi2  Yong Chen2  Shinichi Matsumoto3  Hirofumi Noguchi3  Teru Okitsu3  Yuichi Takeno4  Eiji Kobayashi4  Jonathan R. T. Lakey5  | |
[1] Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, Tokyo 113-8613 Japan;Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan;Department of Transplant Surgery, Kyoto University Hospital, 54 Seigoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan;Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan;Human Pancreatic Islet Transplant Program, University of Alberta, Alberta T2N 4N1, Canada; | |
关键词: Islets; Simian virus 40 large T antigen; Coculture; | |
DOI : 10.3727/000000006783981882 | |
received in 2005-08-27, accepted in 2005-09-30, 发布年份 2006 | |
来源: Sage Journals | |
【 摘 要 】
Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.
【 授权许可】
Unknown
© 2006 Cognizant Comm. Corp.
【 预 览 】
Files | Size | Format | View |
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RO202212200030000ZK.pdf | 6778KB | download |
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