Development of a New Method to Induce Angiogenesis at Subcutaneous Site of Streptozotocin-Induced Diabetic Rats for Islet Transplantation

Cell Transplantation

Article

Wanxing Cui¹  Yoshiyuki Kawakami¹  Yoko Misawa¹  Akira Satake²  Meirigeng Qi²  Yuanjun Gu²  Hiroshi Hori²  Masaaki Miyamoto²  Masumi Nozawa²  Appakaalai N. Balamurugan²  Kazutomo Inoue²  Yasuhiko Tabata²  Natsuki Nagata²  Maki Toma² 

[1] Department of Surgical Basic Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan;Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan;

关键词: Angiogenesis;    Subcutaneous site;    Basic fibroblast growth factor (bFGF);    Collagen sponge;   

DOI  :  10.3727/000000001783986693

来源: Sage Journals

【 摘 要 】

The subcutaneous space is a potential site for clinical islet transplantation. Even though there are several advantages, poor blood supply at this site mainly causes failure of islet survival. In this study, angiogenesis was induced in advance at the diabetic rats subcutis for islet transplantation by implanting a polyethylene terephthalate (PET) mesh bag containing gelatin microspheres incorporating basic fibroblast growth factor (bFGF) (MS/bFGF) and a collagen sponge. The bFGF was incorporated into gelatin microspheres for controlled release of bFGF. As controls, a PET mesh bag with or without either collagen sponges or MS/bFGF was implanted at the subcutaneous site of diabetic rats. Macroscopic and microscopic examinations revealed the formation of capillary network in and around the PET mesh bag containing MS/bFGF and collagen sponges 7 days after implantation when compare with other control groups. When tissue hemoglobin level was also measured, a significantly high level of hemoglobin amount was observed compared with that of control groups. When allogeneic islets mixed with 5% agarose were transplanted into the prevascularized rat subcutis, normoglycemia was maintained for more than 40 days, while other control groups were ineffective. This study demonstrated that combination of gelatin microspheres incorporating bFGF and collagen sponges enabled the mesh to induce neovascularization even at the subcutaneous site of streptozotocin-induced diabetic rats, resulting in improved function of islet transplantation.

【 授权许可】

Unknown   
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