Dystrophin Expression following the Transplantation of Normal Muscle Precursor Cells Protects mdx Muscle from Contraction-Induced Damage

Cell Transplantation

Article

Nicolas Dumont¹  Jérome Frenette¹  Claude H. Côté¹  Simon P. Quenneville²  Joel Rousseau²  Jacques P. Tremblay²  Carl Lebel² 

[1] Unité CRML, Centre de recherche du CHUL, CHUQ, Faculté de médecine, Université Laval, Ste-Foy, QC, Canada;Unité de recherche de recherche en Génétique Humaine, Centre de recherche de CHUL, CHUQ, Faculté de médecine, Université Laval, Sainte-Foy, Québec, Canada;

关键词: Duchenne muscular dystrophy;    mdx;    Muscle precursor cell transplantation;    Muscle contraction;   

DOI  :  10.3727/096368910X4863235

 received in 2009-05-29, accepted in 2010-01-08,  发布年份 2010

来源: Sage Journals

【 摘 要 】

Duchenne muscular dystrophy (DMD) is the most frequent muscular dystrophy. Currently, there is no cure for the disease. The transplantation of muscle precursor cells (MPCs) is one of the possible treatments, because it can restore the expression of dystrophin in DMD muscles. In this study, we investigated the effects of myoblasts injected with cardiotoxin on the contractile properties and resistance to eccentric contractions of transplanted and nontransplanted muscles. We used the extensor digitorum longus (EDL) as a model for our study. We conclude that the sole presence of dystrophin in a high percentage of muscle fibers is not sufficient by itself to increase the absolute or the specific force in the EDL of transplanted mdx muscle. This lack of strength increase may be due to the extensive damage that was produced by the cardiotoxin, which was coinjected with the myoblasts. However, the dystrophin presence is sufficient to protect muscle from eccentric damage as indicated by the force drop results.

【 授权许可】

Unknown   
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