The force-temperature relationship in healthy and dystrophic mouse diaphragm; implications for translational study design.

Frontiers in Physiology

Jenna E Stangland¹  Paul M. L. Janssen¹  Jill A Rafael-Fortney¹  Ben D Canan¹  Jason D Murray¹  Neha eRastogi¹  Christopher D Martin¹ 

[1] Ohio State University;

关键词: Physiology;    mdx;    Contraction;    Duchenne muscular dystrophy;    Muscle strips;   

DOI  :  10.3389/fphys.2012.00422

来源: DOAJ

【 摘 要 】

In the field of muscular dystrophy, striated muscle function is often assessed in vitro in dystrophin-deficient mdx mice in order to test the impact of a potential treatment strategy.Although many past studies have assessed diaphragm contractile function at or near room temperature, the diaphragm performs in vivo at 37ºC.To improve translation of bench-top results to possible clinical application, we studied temperature-dependence of contractile performance in wild-type (C57BL/10) and mdx muscle strips at temperatures from 25 to 37ºC.Maximal tetanic force in wild-type muscles was higher at 37 ºC (198±11 vs. 155±9 mN/mm2 at 25ºC), while the difference between wild-type and mdx was extremely similar: wild-type muscles produced 45.9% and 45.1% more force at 25 and 37ºC respectively.At 37ºC twitch contraction kinetics and 50% rise time to tetanic plateau were slower in mdx diaphragm.A fatigue/injury protocol indicated two-fold fatigue/contraction induced force deficit in mdx muscles.We conclude that assessment of diaphragm muscle strips can be reliably and reproducibly performed at 37 ºC.

【 授权许可】

Unknown