Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Micromachines

Adrian Martinez-Rivas¹  Génesis K. González-Quijano²  Sergio Proa-Coronado³  Childérick Séverac⁴  Etienne Dague⁵ 

[1] CIC, Instituto Politécnico Nacional (IPN), Av. Juan de Dios Bátiz S/N, Nueva Industrial Vallejo, 07738 Mexico City, Mexico;CONACYT-CNMN, Instituto Politécnico Nacional (IPN), Av. Luis Enrique Erro s/n, Nueva Industrial Vallejo, 07738 Mexico City, Mexico;ENCB, Instituto Politécnico Nacional (IPN), Av. Wilfrido Massieu, Unidad Adolfo López Mateos, 07738 Mexico City, Mexico;ITAV-CNRS, Université de Toulouse, CNRS, Toulouse, France;LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France;

关键词: cell patterning and manipulation;    mammalian and bacterial cells;    micro-nanofabrication;    microfluidics;    organs-on-chips (OOC);    biomedical microelectromechanical systems (bioMEMS);    point-of-care (POC);    soft lithography;   

DOI  :  10.3390/mi8120347

来源: DOAJ

【 摘 要 】

Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalized medicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.

【 授权许可】

Unknown