Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars

Biosensors

Francisco J. Ortega²  Mar໚-José Bañuls²  Francisco J. Sanza¹  Rafael Casquel¹  Mar໚ Fe Laguna¹  Miguel Holgado¹  David López-Romero³  Carlos A. Barrios³  Ángel Maquieira² 

[1]Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain

[2] E-Mails:

[3]Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain

[4] E-Mails:

[5]Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de Madrid, ETSI de Telecomunicación, Ciudad Universitaria s/n, Madrid 28040, Spain

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关键词: micro-nanofabrication;    SU-8;    optical interrogation;    label-free nanobiosensing;    gestrinone;    immunoassay affinity constant determination;   

DOI  :  10.3390/bios2030291

来源: mdpi

【 摘 要 】

In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (K_D = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.