An Active Microscaffold System with Fluid Delivery and Stimulation/Recording Functionalities for Culturing 3-D Neuronal Networks

BioMEMS;SU-8;Microscaffold;Multielectrode array;3D;Microfluidics;Microelectrodes;Neuronal networks;3D MEA;3D neuronal networks;MEA

Rowe, Laura Elizabeth ; Electrical and Computer Engineering

University:Georgia Institute of Technology

Department:Electrical and Computer Engineering

关键词: BioMEMS;    SU-8;    Microscaffold;    Multielectrode array;    3D;    Microfluidics;    Microelectrodes;    Neuronal networks;    3D MEA;    3D neuronal networks;    MEA;   

Others  :  https://smartech.gatech.edu/bitstream/1853/14542/1/rowe_laura_e_200705_phd.pdf

美国|英语

来源: SMARTech Repository

【 摘 要 】

An Active Microscaffold System with Fluid Delivery and Stimulation/Recording Functionalities for Culturing 3-D Neuronal NetworksLaura Elizabeth Rowe215 PagesDirected by Dr. A. Bruno FrazierAn active microscaffold system with fluid delivery and electrical stimulation/recording functionalities for 3-D neuronal culture studies is presented.The microscaffolds presented in this dissertation consist of an array of microfabricated towers with integrated microfluidic channels, fluid ports, and electrodes.The microfluidic channels and ports allow for perfusion of nutrients, gas exchange, and biochemical control of the extracellular environment throughout the 3-D culture, while the electrodes allow for active stimulation/recording of the 3-D neuronal network.In essence, the microscaffold serves as an artificial circulatory system to enable 3-D in vitro growth and proliferation of re-aggregate neuronal cell cultures.Increased cell survival on microscaffolds with nutrient perfusion at 14 and 21 days in vitro (DIV) is presented.Additionally, the microtower scaffold is built upon a substrate that is compatible with the Multi Channel Systems preamplifier setup to enable electrical stimulation/recording of the cultured network in a 3-D mutilelectrode array (MEA) environment.Impedance measurements on the functioning microtower electrodes were obtained.The overall goal of this research was to develop a BioMEMS technology to provide neuroscientists with a better investigative tool for studying 3-D in vitro neuronal networks than is currently available.