Structurally Related Monoterpenes p-Cymene, Carvacrol and Thymol Isolated from Essential Oil from Leaves of Lippia sidoides Cham. (Verbenaceae) Protect Mice against Elastase-Induced Emphysema

Molecules

Marina Guerreiro¹  Fernanda R. Santana¹  Ellen Games¹  Emerson A. de Oliveira¹  Carla M. Prado²  João Henrique G. Lago³  Fernanda D.T.Q.S. Lopes⁴  Clarice R. Olivo⁴  Mílton A. Martins⁴  Nathalia M. Pinheiro⁴  Iolanda F.L.C. Tibério⁴ 

[1] Department of Biological Science, Universidade Federal de São Paulo, Diadema 09913-030, Brazil;Department of Bioscience, Federal University of São Paulo, Campus Baixada Santista, Santos 11015-020, SP, Brazil;Department of Exact Science and Earth, Universidade Federal de São Paulo, Diadema 09913-030, Brazil;Department of Medicine, School of Medicine, Universidade de São Paulo, São Paulo 01246903, SP, Brazil;

关键词: COPD;    elastase-induced emphysema;    monoterpenes;    p-cymene;    carvacrol;    thymol;    lung inflammation;   

DOI  :  10.3390/molecules21101390

来源: DOAJ

【 摘 要 】

Background: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and inflammation. Natural products, such as monoterpenes, displayed anti-inflammatory and anti-oxidant activities and can be used as a source of new compounds to COPD treatment. Our aim was to evaluate, in an elastase-induced pulmonary emphysema in mice, the effects of and underlying mechanisms of three related natural monoterpenes (p-cymene, carvacrol and thymol) isolated from essential oil from leaves Lippia sidoides Cham. (Verbenaceae). Methods: Mices received porcine pancreatic elastase (PPE) and were treated with p-cymene, carvacrol, thymol or vehicle 30 min later and again on 7th, 14th and 28th days. Lung inflammatory profile and histological sections were evaluated. Results: In the elastase-instilled animals, the tested monoterpenes reduced alveolar enlargement, macrophages and the levels of IL-1β, IL-6, IL-8 and IL-17 in bronchoalveolar lavage fluid (BALF), and collagen fibers, MMP-9 and p-65-NF-κB-positive cells in lung parenchyma (p < 0.05). All treatments attenuated levels of 8-iso-PGF2α but only thymol was able to reduced exhaled nitric oxide (p < 0.05). Conclusion: Monoterpenes p-cymene, carvacrol and thymol reduced lung emphysema and inflammation in mice. No significant differences among the three monoterpenes treatments were found, suggesting that the presence of hydroxyl group in the molecular structure of thymol and carvacrol do not play a central role in the anti-inflammatory effects.

【 授权许可】

Unknown