The essential oil from Lippia gracilis Schauer, Verbenaceae, in diabetic rats

Revista Brasileira de Farmacognosia

Matos, Francisco J. A.¹  Universidade Federal do Rio Grande do Norte, Natal, Brasil¹  Melo, Maria C. N. de¹  Silva, Sônia L.¹  Guimarães, Sérgio B.¹  Silva, Silvia F. R.¹  Carvalho, Cibele B. M.¹  Andrade, Vânia S.¹  M. Neto, Renato¹  Pessoa, Otília D. L.¹  Universidade de Fortaleza, Fortaleza, Brasil¹  Vasconcelos, Paulo R. L.¹  Universidade Federal do Ceará, Fortaleza, Brasil¹ 

关键词: Lippia gracilis Schauer;    Verbenaceae;    alecrim-de-tabuleiro;    monoterpenes;    carvacrol;    p-cymene;    γ-terpinene.;   

DOI  :  10.1590/S0102-695X2010000200021

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

The essential oil from Lippia gracilis Schauer (Verbenaceae) leaves was examined by GC and GC-MS. Fifteen constituents were identified. Carvacrol, p-cymene and γ-terpinene were found to be the major components. In the in vitro study, 5% solution of the Lippia gracilis Schauer oil presented antibacterial activity against Staphylococcus aureus isolated from diabetic patients with infected ulcers. The study evaluated the antibacterial activity of the 5% solution of the Lippia gracilis Schauer oil on the experimental model of diabetic adult male albino Wistar rats with leaft pelvic limb infected by Staphylococcus aureus strain. In this experiment, 28 diabetic Wistar rats were used, randomly distributed in four different groups of seven rats, (G1-white; G2-negative control; G3-positive control and G4-test). When comparing group G4 with G3, it was observed that the 5% solution presented a reduced CFU/mL level showing the antibacterial effect of the oil 24 hours after the administration of the inoculum (S .aureus without Lippia gracilis Schauer 108 ±313 versus S.aureus with Lippia gracilis Schauer 13.28±4.03). The results were expressed as mean±S.E.M. One-way analysis of the variance (ANOVA) was used. The differences between the minimum inhibitory concentration in vitro test were determined by the Tukey test (p<0.05). The Newman-Keuls test with level of significance (p<0.05) was used to measure the results in vivo. The findings have shown that 5% solution of the Lippia gracilis Schauer oil presented antibacterial activity in vitro and in vivo.

【 授权许可】

Unknown