Long non-coding RNA profiling of pediatric Medulloblastoma

BMC Medical Genomics

Varun Kesherwani¹  Mamta Shukla²  Shantaram S. Joshi²  J. Graham Sharp²  Nagendra K. Chaturvedi³  Don W. Coulter³ 

[1] Child Health Research Institute, University of Nebraska Medical Center;Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center;Department of Pediatrics, Hematology and Oncology Division, University of Nebraska Medical Center;

关键词: Long non-coding RNA;    Pediatric Medulloblastoma;    Cancer biomarkers;    Gene expression and pathways;    Therapeutic targets;   

DOI  :  10.1186/s12920-020-00744-7

来源: DOAJ

【 摘 要 】

Abstract Background Medulloblastoma (MB) is one of the most common malignant cancers in children. MB is primarily classified into four subgroups based on molecular and clinical characteristics as (1) WNT (2) Sonic-hedgehog (SHH) (3) Group 3 (4) Group 4. Molecular characteristics used for MB classification are based on genomic and mRNAs profiles. MB subgroups share genomic and mRNA profiles and require multiple molecular markers for differentiation from each other. Long non-coding RNAs (lncRNAs) are more than 200 nucleotide long RNAs and primarily involve in gene regulation at epigenetic and post-transcriptional levels. LncRNAs have been recognized as diagnostic and prognostic markers in several cancers. However, the lncRNA expression profile of MB is unknown. Methods We used the publicly available gene expression datasets for the profiling of lncRNA expression across MB subgroups. Functional analysis of differentially expressed lncRNAs was accomplished by Ingenuity pathway analysis (IPA). Results In the current study, we have identified and validated the lncRNA expression profile across pediatric MB subgroups and associated molecular pathways. We have also identified the prognostic significance of lncRNAs and unique lncRNAs associated with each MB subgroup. Conclusions Identified lncRNAs can be used as single biomarkers for molecular identification of MB subgroups that warrant further investigation and functional validation.

【 授权许可】

Unknown