Molecular species of Diaphanes sp from Nusa Kambangan Island as a source of luciferase molecular marker on cancer diagnostic

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Puspitaningrum, R.^1 ; Rustam, Y.^1 ; Handayani, T.^1 ; Mudjihartini, N.^2 ; Junihadi, G.D.^3

Department of Biology, Faculty of Math and Natural Sciences UNJ, Rawamangun Muka, Jakarta

13220, Indonesia^1

Biochemistry and Molecular Biology FKUI, Jakarta

10430, Indonesia^2

Bandung Institute of Technology, Jl. Ganesha No.10, Lb.Silingawi, Coblong, Kota Bandung, Jawa Barat

0132, Indonesia^3

关键词: Cancer biomarkers;    Cancer diagnostics;    Descriptive analysis;    Gene sequences;    Luciferase genes;    Molecular marker;    Molecular species;    Native species;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012103/pdf
DOI  :  10.1088/1757-899X/434/1/012103

来源: IOP

【 摘 要 】

The luciferase, an important element, is currently being developed as an early detection for cancer biomarker. This study aims to obtain the gene sequence for luciferase and discover the percentage of similarity between the luciferase gene from Diaphanes javanus Gorham and gene bank reference fireflies. The study employed a descriptive analysis method. The results of the isolation, restriction and amplification of the luciferase gene produced a single band and nucleotide readings. The luciferase contig sequence for Diaphanes sp. was compared to the with the gene bank reference sequence. The highest similarity of the luciferase gene in Diaphanes sp was showing high similarity. The similarity rate can help to determine the direction of the development of the Diaphones sp luciferase gene in native species of Indonesia.