Aspergillus Diversity Associated with Fungal Diseases on Fish with Molecular Based

4th International Conference on Tropical and Coastal Region Eco Development

生态环境科学;经济学

Sarjito^1 ; Condro Haditomo, Alfabetian Harjuno^1 ; Sabdaningsih, Aninditia^2 ; Desrina^1 ; Prayitno, Slamet Budi^1

Aquaculture Study Program, Aquaculture Department, Fisheries and Marine Science Faculty, Diponegoro University, Jl. Prof. Soedarto SH, Tembalang, Semarang

50275, Indonesia^1

Department of Aquatic Science, Fisheries and Marine Science Faculty, DiponegoroUniversity, Prof. Sudharto, Tembalang, Semarang, Indonesia^2

关键词: 16S rDNA analysis;    Aspergillus fumigatus;    Aspergillus niger;    Clinical signs;    Fish cultures;    Fungal disease;    Gene sequences;    Moribund fishes;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/246/1/012035/pdf
DOI  :  10.1088/1755-1315/246/1/012035

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Fungal diseases are frequently occur in fish culture. The aim of this research was to find out the diversity of Aspergillusassociated with fungal diseases in catfish and Tilapia based on 16S rDNA gene sequences in central Java Indonesia. The combination between exploratory in the field and experiment, method were applied. In order to find out the Aspergillus prevalence, 48 fish were collected from fish pond of Demak, Klaten and Semarang Regency. Based on the clinical sign, 24 moribund fish were chosen for fungus isolation. As a result, 21 fungi isolates (FTD01-FTD05; FTK01-FTK08; FCB01-FCB08) were gained from external wounds of fish with Sabouraud Dextrose Agar (SDA) medium. Based on the Postulate Koch result showed that three isolates (FTD03, FTK07 and FCB01) that were caused 20 - 80% of fish get sick and mortal. On the basis of sequence 16S rDNA analysis, the result showed that FTK01, FTK07, and FCB01 were closely related toAspergillus flavus (100%); Aspergillus niger (71%)and Aspergillus fumigatus (77%) respectively.