Qualitative and quantitative analysis of Baltic phytoplankton pigments

Oceanologia

关键词: Chlorophylls;    Photosynthetic carotenoids;    Photoprotecting carotenoids;    HPLC;    Gulf of Gdañ sk;   

DOI  :  

来源: DOAJ

【 摘 要 】

The paper presents the results of research into the pigment composition ofseawater samples taken from various depths in the Gulf of Gdanskin April and September 1999. Pigments were separated by RP-HPLC, and identification wasconfirmed by co-injection with reference standards and on-line diode arrayspectra. The following groups of pigments were identified: chlorophylls: a,b, c₁ + c₂; photosynthetic carotenoids (PSC) - peridinin, fucoxanthin,alfa-carotene; photoprotecting carotenoids (PPC) - diadinoxanthin,alloxanthin, zeaxanthin, lutein, neoxanthin, violaxanthin and beta-carotene. Quantitative and qualitative diversityin pigment characteristics were observed in both seasons, though lutein and alfa-carotene were notidentified in the September samples. The respective April and September concentrations of thefunctional groups of pigments were: total chlorophylls content 0.47-104.25and 0.57-13.66 µg dm^–3; PSC 0.07-21.23 and 0.02-1.56 µg dm^–3;PPC 0.05-14.08 and 0.04-1.79 µg dm^–3. Within the PSC group,peridinin and fucoxanthin were dominant in the April samples, but only fucoxanthinin the September ones. Among the PPCs, diadinoxanthin and alloxanthin were dominant in April, zeaxanthin in September. Photosynthetic and photoprotecting carotenoids display a linear correlation withchlorophyll alfa content in all the samples.

【 授权许可】

Unknown