Biosynthesis of flower-shaped Au nanoclusters with EGCG and their application for drug delivery

Journal of Nanobiotechnology

Mingyuan Zou¹  Shichao Wu¹  Fanghong Luo¹  Ting Wu¹  Peilan Xu¹  Xiangrui Yang¹  Jianghua Yan¹ 

[1] Cancer Research Center, Medical College, Xiamen University;

关键词: Biosynthesis;    Au nanoclusters;    Drug delivery;    Targeting;    Dual drug;   

DOI  :  10.1186/s12951-018-0417-3

来源: DOAJ

【 摘 要 】

Abstract Background In the last decade, the biosynthesis of metal nanoparticles using organisms have received more and more considerations. However, the complex composition of organisms adds up to a great barrier for the characterization of biomolecules involved in the synthesis process and their biological mechanisms. Results In this research, we biosynthesized a kind of flower-shaped Au nanoclusters (Au NCs) using one definite component—epigallocatechin gallate (EGCG), which was the main biomolecules of green tea polyphenols. Possessing good stability for 6 weeks and a size of 50 nm, the Au NCs might be a successful candidate for drug delivery. Hence, both methotrexate (MTX) and doxorubicin (DOX) were conjugated to the Au NCs through a bridge of cysteine (Cys). The introduction of MTX provided good targeting property for the Au NCs, and the conjugation of DOX provided good synergistic effect. Then, a novel kind of dual-drug loaded, tumor-targeted and highly efficient drug delivery system (Au-Cys-MTX/DOX NCs) for combination therapy was successfully prepared. The TEM of HeLa cells incubated with Au-Cys-MTX/DOX NCs indicated that the Au-Cys-MTX/DOX NCs could indeed enter and kill cancer cells. The Au-Cys-MTX/DOX NCs also possessed good targeting effect to the FA-receptors-overpressed cancer cells both in vitro and in vivo. Importantly, the Au-Cys-MTX/DOX NCs resulted in an excellent anticancer activity in vivo with negligible side effects. Conclusions These results suggest that the biosynthesized Au-Cys-MTX/DOX NCs could be a potential carrier with highly efficient anticancer properties for tumor-targeted drug delivery.

【 授权许可】

Unknown