Antioxidant peptides derived from the hydrolyzate of purple sea urchin (Strongylocentrotus nudus) gonad alleviate oxidative stress in Caenorhabditis elegans

Journal of Functional Foods

Weizhang Jia¹  Zebo Huang²  Ming Liang²  Xuesong Yu²  Qiong Cheng²  Chung Wah Ma³  Sixu Zhao³  Qiong Peng³  Xiquan Yin³ 

[1] Guangdong Province Key Laboratory for Biotehnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, China;School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China;;Center for Bioresources and Drug Discovery &

关键词: Antioxidant peptide;    Oxidative stress;    Reactive oxygen species;    Purple sea urchin Strongylocentrotus nudus;    Caenorhabditis elegans;   

DOI  :  

来源: DOAJ

【 摘 要 】

The papain hydrolyzate prepared from purple sea urchin (Strongylocentrotus nudus) gonad exerts significant antioxidant activity. Through ultrafiltration, gel filtration and high performance liquid chromatography, the active fractions from the hydrolyzate were screened by alleviating paraquat-induced oxidative stress in Caenorhabditis elegans, and the peptide components were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two antioxidant peptides SnP7 (AAVPSGASTGIYEALELR, 1805.03 Da) and SnP10 (NPLLEAFGNAK, 1173.34 Da) exhibit significant antioxidant capacity, which reduce reactive oxygen species (ROS) level and the expression of superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2) in oxidation-damaged nematodes. Further studies indicated that SnP7 and SnP10 induce DAF-16 nuclear translocation and the expression of stress-related genes such as sod-3. RNA interference (RNAi) suggested that peptide-induced enhancement of resistance is dependent on DAF-16. Taken together, S. nudus antioxidant peptides might act through activate DAF-16 signaling pathway, and induce the expression of DAF-16 target genes that enhance antioxidant potential of organisms.

【 授权许可】

Unknown