Antioxidant peptides from Antarctic Krill (Euphausia superba) hydrolysate: Preparation, identification and cytoprotection on H2O2-induced oxidative stress

Journal of Functional Foods

Bin Wang¹  Peng Wang²  Chang-Feng Chi³  Yu-Mei Wang⁴  Yue-Zhen Wang⁴  Wen-Hao Zhao⁴  Yu-Qin Zhao⁴ 

[1] Corresponding authors.;College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China;Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China;

关键词: Antarctic Krill (Euphausia superba);    Antioxidant peptide;    Radical scavenging activity;    Oxidative stress;    Cytoprotection;   

DOI  :  

来源: DOAJ

【 摘 要 】

The aim of this study was to purify and identify antioxidant peptides from Antarctic Krill hydrolysate and elucidate their cytoprotection mechanisms on H2O2-induced oxidative stress in Chang liver cells. Using ultrafiltration and chromatographic methods, twelve peptides were purified from the Antarctic Krill hydrolysate and identified as AEK, VEK, VEKT, AEKTR, IEN, VEKGK, LKPGN, IEKG, LQP, ATH, IEKT, and IDSQ. LKPGN and LQP exhibited high scavenging activities on HO·, DPPH·, and O2−·. Moreover, LKPGN and LQP can protect Chang liver cells against H2O2-induced oxidative stress by increasing cell viability and decreasing the apoptosis rate. The mechanism indicated that LKPGN and LQP could concentration-dependently increase the antioxidant enzyme (SOD and GSH-PX) levels to scavenge excess ROS, increase mitochondrial membrane potential, and decrease DNA damage and MDA content. Therefore, LKPGN and LQP from Antarctic Krill could serve as promising candidates applied in health-promoting products.

【 授权许可】

Unknown