Photoinactivation of Yeast and Biofilm Communities of Candida albicans Mediated by ZnTnHex-2-PyP4+ Porphyrin

Journal of Fungi

Martha S. Ribeiro¹  Anderson Z. Freitas¹  Bruno L. Raposo²  Paulo E. Cabral Filho²  Adriana Fontes²  Sueden O. Souza²  Beate S. Santos³  Danielle P. C. Macêdo³  Regina C. B. Q. Figueiredo⁴  José F. Sarmento-Neto⁵  Júlio S. Rebouças⁵ 

[1] Centro de Lasers e Aplicações, Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN), São Paulo 05508-000, SP, Brazil;Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil;Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife 50740-520, PE, Brazil;Departamento de Microbiologia, Instituto Aggeu Magalhães—Fundação Oswaldo Cruz (IAM-FIOCRUZ), Recife 50740-465, PE, Brazil;Departamento de Química, Universidade Federal da Paraíba, João Pessoa 58051-900, PB, Brazil;

关键词: antimicrobial photodynamic inactivation;    blue light;    fungi;    photodynamic therapy;    Zn(II) porphyrin;   

DOI  :  10.3390/jof8060556

来源: DOAJ

【 摘 要 】

Candida albicans is the main cause of superficial candidiasis. While the antifungals available are defied by biofilm formation and resistance emergence, antimicrobial photodynamic inactivation (aPDI) arises as an alternative antifungal therapy. The tetracationic metalloporphyrin Zn(II) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin (ZnTnHex-2-PyP⁴⁺) has high photoefficiency and improved cellular interactions. We investigated the ZnTnHex-2-PyP⁴⁺ as a photosensitizer (PS) to photoinactivate yeasts and biofilms of C. albicans strains (ATCC 10231 and ATCC 90028) using a blue light-emitting diode. The photoinactivation of yeasts was evaluated by quantifying the colony forming units. The aPDI of ATCC 90028 biofilms was assessed by the MTT assay, propidium iodide (PI) labeling, and scanning electron microscopy. Mammalian cytotoxicity was investigated in Vero cells using MTT assay. The aPDI (4.3 J/cm²) promoted eradication of yeasts at 0.8 and 1.5 µM of PS for ATCC 10231 and ATCC 90028, respectively. At 0.8 µM and same light dose, aPDI-treated biofilms showed intense PI labeling, about 89% decrease in the cell viability, and structural alterations with reduced hyphae. No considerable toxicity was observed in mammalian cells. Our results introduce the ZnTnHex-2-PyP⁴⁺ as a promising PS to photoinactivate both yeasts and biofilms of C. albicans, stimulating studies with other Candida species and resistant isolates.

【 授权许可】

Unknown