Biogenic Silver Nanoparticles Decorated with Methylene Blue Potentiated the Photodynamic Inactivation of Pseudomonas aeruginosa and Staphylococcus aureus

Pharmaceutics

Madhu Dyavaiah¹  V.T. Anju¹  HelalF. Al-Harthi²  AliH. Bahkali²  Asad Syed²  Thamanna R. Y³  Busi Siddhardha³  Paramanantham Parasuraman³  Chitra Shaji⁴  Alok Sharan⁴ 

[1] Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry 605014, India;Department of Botany and Microbiology, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451, Saudi Arabia;Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India;Department of Physics, School of Physical, Chemical & Applied Sciences, Pondicherry University, Puducherry 605014, India;

关键词: multidrug resistance;    antimicrobial photodynamic inactivation;    silver nanoparticles;    methylene blue;    anti-biofilm;   

DOI  :  10.3390/pharmaceutics12080709

来源: DOAJ

【 摘 要 】

The persistence of multidrug resistance among microorganisms has directed a mandate towards a hunt for the development of alternative therapeutic modalities. In this context, antimicrobial photodynamic therapy (aPDT) is sprouted as a novel strategy to mitigate biofilms and planktonic cells of pathogens. Nanoparticles (NPs) are reported with unique intrinsic and antimicrobial properties. Therefore, silver NPs (AgNPs) were investigated in this study to determine their ability to potentiate the aPDT of photosensitizer against Staphylococcus aureus and Pseudomonas aeruginosa. Biologically synthesized AgNPs were surface coated with methylene blue (MB) and studied for their aPDT against planktonic cells and biofilms of bacteria. The nano-conjugates (MB-AgNPs) were characterized for their size, shape and coated materials. MB-AgNPs showed significant phototoxicity against both forms of test bacteria and no toxicity was observed in the dark. Moreover, activity of MB-AgNPs was comparatively higher than that of the free MB, which concludes that MB-AgNPs could be an excellent alternative to combat antibiotic resistant bacteria.

【 授权许可】

Unknown