Synaptic plasticity during recovery from permanent occlusion of the middle cerebral artery

Neurobiology of Disease

Anna Tortiglione¹  Silvia Rossi²  Chiara Prosperetti²  Barbara Picconi³  Paolo Calabresi³  Diego Centonze³  Giorgio Bernardi⁴  Valentina De Chiara⁴ 

[1] Corresponding authors. D. Centonze is to be contacted at Clinica Neurologica, Dipartimento di Neuroscienze, Università Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. Fax: [phone]. P. Calabresi, Clinica Neurologia, Università di Perugia, Ospedale Silvestrini, S. Andrea delle Fratte, 06156 Perugia, Italy. Fax: [phone].;Istituto di Ricovero e Cura a Carattere Scientifico Fondazione Santa Lucia, 00179 Rome, Italy;Clinica Neurologica, Dipartimento di Neuroscienze, Università Tor Vergata, 00133 Rome, Italy;Istituto di Ricovero e Cura a Carattere Scientifico Fondazione Santa Lucia, 00179 Rome, Italy;

关键词: AMPA receptors;    Brain ischemia;    Electrophysiology;    Glutamate;    NMDA receptors;    pMCAO;   

DOI  :  

来源: DOAJ

【 摘 要 】

Synaptic rearrangements in the peri-infarct regions are believed to contribute to the partial recovery of function that takes place after stroke. Here, we performed neurophysiological recordings from single neurons of rats with permanent occlusion of the middle cerebral artery (pMCAO) during the resolution of their neurological deficits. Our results show that complex and dynamic changes of glutamate transmission in the peri-infarct area parallel the recovery from brain infarct. We have observed that frequency and duration of spontaneous glutamate-mediated synaptic events were markedly increased in striatal neurons during the early phase of the recovery (3 days after pMCAO), due to potentiation of both NMDA (N-methyl-d-aspartate) and non-NMDA receptor-mediated transmission. In the late phase of recovery (7 days after pMCAO), glutamate transmission was still enhanced because of a selective facilitation of non-NMDA receptor-mediated transmission. Spiny projection neurons but not aspiny interneurons underwent detectable changes of synaptic excitability in the striatum following pMCAO, indicating that the process of neuronal adaptation after focal brain ischemia is cell-type-specific. Our results provide a synaptic correlate of the long-lasting brain hyperexcitability mediating recovery described with noninvasive neurophysiological approaches.

【 授权许可】

Unknown