Biotechnology for Biofuels | |
High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae | |
Moo-Young Jung1  Min-Kyu Oh2  Jung Hun Lee2  | |
[1] CJ Research Institute of Biotechnology;Department of Chemical and Biological Engineering, Korea University; | |
关键词: 1, 3-PDO; Klebsiella pneumoniae; Glycerol; Byproducts; Glycerol assimilation pathway; Co-substrate; | |
DOI : 10.1186/s13068-018-1100-5 | |
来源: DOAJ |
【 摘 要 】
Abstract Background Glycerol is a major byproduct of the biodiesel industry and can be converted to 1,3-propanediol (1,3-PDO) by microorganisms through a two-step enzymatic reaction. The production of 1,3-PDO from glycerol using microorganisms is accompanied by formation of unwanted byproducts, including lactate and 2,3-butanediol, resulting in a low-conversion yield. Results Klebsiella pneumoniae was metabolically engineered to produce high-molar yield of 1,3-PDO from glycerol. First, the pathway genes for byproduct formation were deleted in K. pneumoniae. Then, glycerol assimilation pathways were eliminated and mannitol was co-fed to the medium. Finally, transcriptional regulation of the dha operon were genetically modified for enhancing 1,3-propanediol production. The batch fermentation of the engineered strain with co-feeding of a small amount of mannitol yielded 0.76 mol 1,3-PDO from 1 mol glycerol. Conclusions Klebsiella pneumoniae is useful microorganism for producing 1,3-PDO from glycerol. Implemented engineering in this study successfully improved 1,3-PDO production yield, which is significantly higher than those reported in previous studies.
【 授权许可】
Unknown