High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae

Biotechnology for Biofuels

Moo-Young Jung¹  Min-Kyu Oh²  Jung Hun Lee² 

[1] CJ Research Institute of Biotechnology;Department of Chemical and Biological Engineering, Korea University;

关键词: 1, 3-PDO;    Klebsiella pneumoniae;    Glycerol;    Byproducts;    Glycerol assimilation pathway;    Co-substrate;   

DOI  :  10.1186/s13068-018-1100-5

来源: DOAJ

【 摘 要 】

Abstract Background Glycerol is a major byproduct of the biodiesel industry and can be converted to 1,3-propanediol (1,3-PDO) by microorganisms through a two-step enzymatic reaction. The production of 1,3-PDO from glycerol using microorganisms is accompanied by formation of unwanted byproducts, including lactate and 2,3-butanediol, resulting in a low-conversion yield. Results Klebsiella pneumoniae was metabolically engineered to produce high-molar yield of 1,3-PDO from glycerol. First, the pathway genes for byproduct formation were deleted in K. pneumoniae. Then, glycerol assimilation pathways were eliminated and mannitol was co-fed to the medium. Finally, transcriptional regulation of the dha operon were genetically modified for enhancing 1,3-propanediol production. The batch fermentation of the engineered strain with co-feeding of a small amount of mannitol yielded 0.76 mol 1,3-PDO from 1 mol glycerol. Conclusions Klebsiella pneumoniae is useful microorganism for producing 1,3-PDO from glycerol. Implemented engineering in this study successfully improved 1,3-PDO production yield, which is significantly higher than those reported in previous studies.

【 授权许可】

Unknown