Release of DNA from Dermanyssus gallinae during the Biting Process

Animals

Rossella Samarelli¹  Elena Circella¹  Nicola Pugliese¹  Antonella Schiavone¹  Antonio Camarda¹  Lucia Potenza²  Maria Assunta Cafiero³  Ilaria Vasco³  Donato Antonio Raele³  Germana Pennuzzi³ 

[1] Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, S.P. per Casamassima, km 3, 70010 Valenzano, Italy;Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino Carlo Bo, Via A. Saffi, 2, 61029 Urbino, Italy;Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20, 71121 Foggia, Italy;

关键词: Dermanyssus gallinae;    laying hens;    ITS;    COI;    nuclear DNA;    mitochondrial DNA;   

DOI  :  10.3390/ani12091084

来源: DOAJ

【 摘 要 】

Dermanyssus gallinae is a hematophagous ectoparasitic mite that usually infests poultry, but is also known for occasionally attacking other animals and humans. It represents a major problem for poultry systems all over the world, with detrimental effects for both production and animal welfare. Despite the significance of D. gallinae, very little is known about the biting process to date. Therefore, this study has aimed to verify if mite DNA is injected into the host skin during the blood meal. Mite DNA has been detected by seminested PCR from infested chicken skin and quantified by real-time PCR. Furthermore, its localization within the host tissue has been checked by fluorescent in situ hybridization. Results showed that a very little amount of D. gallinae DNA can be released by mites, suggesting that the latter do not introduce whole or partially destroyed cells into the host, but rather it injects traces of nucleic acids, possibly together with merocrine secretions.

【 授权许可】

Unknown