Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam

Parasite

and Y. Nakai³  Y. Fukuda³  T. Itagaki²  M. Ichikawa²  S. Amer⁴  S. Nguyen¹ 

[1] Department of Parasitology, Central Viet Nam Veterinary Institute,km4 Dong De Street,Nha Trang,Viet Nam;Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550,Japan;Laboratory of Sustainable Environmental Biology, Field Centre studies, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711,Japan;Department of Zoology, Faculty of Science, Kafr El Sheikh University,Kafr El Sheikh 33516,Egypt

关键词: Vietnam;    NDI;    COI;    ITS;    parthenogenesis;    hybridization;    Fasciola hepatica;    Fasciola gigantica;   

Others  :  808838
DOI  :  doi:10.1051/parasite/2012191085

 received in 2010-09-28, accepted in 2011-10-17,  发布年份 2012

【 摘 要 】

Fasciola spp. were collected from naturally infected cattle at a local abattoir of Khanh Hoa province, Vietnam, for morphological and genetic investigations. Microscopic examination detected no sperm cells in the seminal vesicles, suggesting a parthenogenetic reproduction of the flukes. Analyses of sequences from the first and second internal transcribed spacers (ITS1 and ITS2) of the ribosomal RNA revealed that 13 out of 16 isolates were of Fasciola gigantica type, whereas three isolates presented a hybrid sequence from F. gigantica and Fasciola hepatica. Interestingly, all the mitochondrial sequences (partial COI and NDI) were of F. gigantica type, suggesting that the maternal lineage of the hybrid form is from F. gigantica. No intra-sequence variation was detected.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2012

【 图 表 】

Fig. 1.

Fig. 2.

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