A Lipophilic Fucoxanthin-Rich Phaeodactylum tricornutum Extract Ameliorates Effects of Diet-Induced Obesity in C57BL/6J Mice

Nutrients

Ulrike Schmid-Staiger¹  Felix Derwenskus²  Clemens Posten³  Andreas Trautmann³  Andreu Palou⁴  Bojan Stojnic⁴  M.Luisa Bonet⁴  Joan Ribot⁴  Andrea Gille⁵  Karlis Briviba⁵ 

[1] Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, Germany;Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, 70569 Stuttgart, Germany;Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Sciences III Bioprocess Engineering, 76131 Karlsruhe, Germany;Laboratory of Molecular Biology, Nutrition and Biotechnology, Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain;Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, 76131 Karlsruhe, Germany;

关键词: Phaeodactylum tricornutum;    microalgae;    fucoxanthin;    eicosapentanoic acid;    obesity;    browning;    brown adipose tissue;   

DOI  :  10.3390/nu11040796

来源: DOAJ

【 摘 要 】

Phaeodactylum tricornutum (P. tricornutum) comprise several lipophilic constituents with proposed anti-obesity and anti-diabetic properties. We investigated the effect of an ethanolic P. tricornutum extract (PTE) on energy metabolism in obesity-prone mice fed a high fat diet (HFD). Six- to eight-week-old male C57BL/6J mice were switched to HFD and, at the same time, received orally placebo or PTE (100 mg or 300 mg/kg body weight/day). Body weight, body composition, and food intake were monitored. After 26 days, blood and tissue samples were collected for biochemical, morphological, and gene expression analyses. PTE-supplemented mice accumulated fucoxanthin metabolites in adipose tissues and attained lower body weight gain, body fat content, weight of white adipose tissue (WAT) depots, and inguinal WAT adipocyte size than controls, independent of decreased food intake. PTE supplementation was associated with lower expression of Mest (a marker of fat tissue expandability) in WAT depots, lower gene expression related to lipid uptake and turnover in visceral WAT, increased expression of genes key to fatty acid oxidation and thermogenesis (Cpt1, Ucp1) in subcutaneous WAT, and signs of thermogenic activation including enhanced UCP1 protein in interscapular brown adipose tissue. In conclusion, these data show the potential of PTE to ameliorate HFD-induced obesity in vivo.

【 授权许可】

Unknown