Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages

Cell Reports

Bailin Wu¹  Luyue Wang²  Wu Wei²  Yuwei Sun³  Zhiyong Liu³  Guangqin Wang³  Zhengnan Luo³  Chao Li³  Tong Zhu⁴ 

[1] Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai 200031, China;University of Chinese Academy of Sciences, Beijing 100049, China;Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China;University of Chinese Academy of Sciences, Beijing 100049, China;

关键词: otocyst;    inner ear;    single-cell RNA-seq;    spiral ganglion neuron;    vestibular ganglion neuron;    Myt1;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Inner ear vestibular and spiral ganglion neurons (VGNs and SGNs) are known to play pivotal roles in balance control and sound detection. However, the molecular mechanisms underlying otic neurogenesis at early embryonic ages have remained unclear. Here, we use single-cell RNA sequencing to reveal the transcriptomes of mouse otic tissues at three embryonic ages, embryonic day 9.5 (E9.5), E11.5, and E13.5, covering proliferating and undifferentiated otic neuroblasts and differentiating VGNs and SGNs. We validate the high quality of our studies by using multiple assays, including genetic fate mapping analysis, and we uncover several genes upregulated in neuroblasts or differentiating VGNs and SGNs, such as Shox2, Myt1, Casz1, and Sall3. Notably, our findings suggest a general cascaded differentiation trajectory during early otic neurogenesis. The comprehensive understanding of early otic neurogenesis provided by our study holds critical implications for both basic and translational research.

【 授权许可】

Unknown