Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics

Cell Reports

Yasmin Eltawil¹  Daniel C. Ellwanger¹  Leon Chen²  Stefan Heller²  Angela H. Ling²  Taha A. Jan²  Alan G. Cheng² 

[1] Department of Otolaryngology-Head and Neck Surgery, University of California San Francisco, San Francisco, CA 94115, USA;Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Palo Alto, CA 94305, USA;

关键词: single-cell RNA-seq;    inner ear;    transitional epithelium;    hair cell progenitor;    regeneration;    sensory cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The utricle is a vestibular sensory organ that requires mechanosensitive hair cells to detect linear acceleration. In neonatal mice, new hair cells are derived from non-sensory supporting cells, yet cell type diversity and mechanisms of cell addition remain poorly characterized. Here, we perform computational analyses on single-cell transcriptomes to categorize cell types and resolve 14 individual sensory and non-sensory subtypes. Along the periphery of the sensory epithelium, we uncover distinct groups of transitional epithelial cells, marked by Islr, Cnmd, and Enpep expression. By reconstructing de novo trajectories and gene dynamics, we show that as the utricle expands, Islr+ transitional epithelial cells exhibit a dynamic and proliferative phase to generate new supporting cells, followed by coordinated differentiation into hair cells. Taken together, our study reveals a sequential and coordinated process by which non-sensory epithelial cells contribute to growth of the postnatal mouse sensory epithelium.

【 授权许可】

Unknown