Apoptosis in the Developing Zebrafish Embryo

Developmental Biology

L.S. Ross¹  L.K. Cole¹ 

[1] Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio, 45701

关键词: cell death;    nervous system;    morphogenesis;    TUNEL;    brain;    olfactory;    visual;    lateral line;    Rohon–Beard;    otocyst;   

DOI  :  10.1006/dbio.2001.0432

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Apoptosisisamajorpartofthenormaldevelopmentofmanyorgansystemsandtissues.Thezebrafish(Daniorerio)hasbecomeausefulmodelforstudyingearlydevelopment,andrecentadvancesintechniquesusedtolabelapoptoticcellshavemadeitpossibletovisualizeapoptoticcellsinthismodelsystem.Wehaveusedtheinsituterminaldeoxynucleotidyltransferase(TdT)-mediateddUTPnick-endlabeling(TUNEL)todescribethetemporalandspatialdistributionofapoptoticcellsduringnormaldevelopmentofthezebrafishembryofrom12to96hpostfertilization.Bycountinglabeledapoptoticcells,wehavedemonstratedtransienthighratesofcelldeathinvariousstructuresduringdevelopment,andwehavecorrelatedthesepeakswithpreviouslydescribeddevelopmentalchangesinthesestructures.Ouranalysishasfocusedonthenervoussystemandassociatedsensoryorgansincludingtheolfactoryorgan,retina,lens,cornea,oticvesicle,laterallineorgans,andRohon–Beardneurons.Apoptosisisalsodescribedinothernon-neuralstructuressuchasthenotochord,somites,muscle,tailbud,andfins.

【 授权许可】

Unknown