Age-dependent differences in myelin basic protein expression in the hippocampus of young, adult and aged gerbils

Laboratory Animal Research

Ji Hyeon Ahn¹  Joon Ha Park¹  Young Joo Lee²  Il Jun Kang³  Jae Chul Lee⁴  Jeong Hwi Cho⁴  Moo-Ho Won⁴  Tae-Kyeong Lee⁴  In Hye Kim⁴  Choong-Hyun Lee⁵  Yong Hwan Jeon⁶  Seongkweon Hong⁷ 

[1] Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, 119 Dandaero;Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University;Department of Food Science and Nutrition, Hallym University;Department of Neurobiology, School of Medicine, Kangwon National University;Department of Pharmacy, College of Pharmacy, Dankook University;Department of Radiology, School of Medicine, Kangwon National University, Kangwon National University Hospital;Department of Surgery, School of Medicine, Kangwon National University, Kangwon National University Hospital;

关键词: Myelin basic protein;    aging;    hippocampus;    gerbil;   

DOI  :  10.5625/lar.2017.33.3.237

来源: DOAJ

【 摘 要 】

Abstract Myelin degeneration is one of the characteristics of aging and degenerative diseases. This study investigated age-related alterations in expression of myelin basic protein (MBP) in the hippocampal subregions (dentate gyrus, CA2/3 and CA1 areas) of gerbils of various ages; young (1 month), adult (6 months) and aged (24 months), using western blot and immunohistochemistry. Western blot results showed tendencies of age-related reductions of MBP levels. MBP immunoreactivity was significantly decreased with age in synaptic sites of trisynaptic loops, perforant paths, mossy fibers, and Schaffer collaterals. In particular, MBP immunoreactive fibers in the dentate molecular cell layer (perforant path) was significantly reduced in adult and aged subjects. In addition, MBP immunoreactive mossy fibers in the dentate polymorphic layer and in the CA3 striatum radiatum was significantly decreased in the aged group. Furthermore, we observed similar age-related alterations in the CA1 stratum radiatum (Schaffer collaterals). However, the density of MBP immunoreactive fibers in the dentate granular cell layer and CA stratum pyramidale was decreased with aging. These findings indicate that expression of MBP is age-dependent and tissue specific according to hippocampal layers.

【 授权许可】

Unknown