Differentiation of human pluripotent stem cells into pancreatic duct-like organoids

STAR Protocols

Sandra Heller¹  Thomas Seufferlein²  Michael Karl Melzer²  Jessica Merkle²  Matthias Meier²  Markus Breunig²  Alexander Kleger²  Meike Hohwieler³ 

[1] Department of Urology, Ulm University, 89081 Ulm, Germany;Department of Internal Medicine I, Ulm University Hospital, 89081 Ulm, Germany;Helmholtz Pioneer Campus, Helmholtz Zentrum München, 85764 München, Germany;

关键词: Cell culture;    Developmental biology;    Stem Cells;    Cell Differentiation;    Organoids;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive.For complete details on the use and execution of this protocol, please refer to Breunig et al. (2021).

【 授权许可】

Unknown