Single tumor imaging with multiple antibodies targeting different antigens

BioTechniques

Girja Shukla¹  Yujing Sun¹  Stephanie C. Pero¹  David Krag¹  Erika Currier²  Giselle Sholler² 

[1] 1Department of Surgery, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA;2Department of Pediatrics, Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VT, USA;

关键词: combination administration;    antibody;    near-infrared fluorescence imaging;    breast cancer;    xenograft;    mouse;   

DOI  :  10.2144/000113855

来源: DOAJ

【 摘 要 】

Antibodies are important drugs for treating cancer and there is strong rationale for using multiple antibodies to improve outcomes. We labeled two breast cancer binding antibodies, anti-ErbB2 and anti-EpCAM, with infrared fluorescence dyes of different wavelengths and determined their in vivo distribution in a breast cancer xenograft model using a near-infrared (NIR) fluorescence imaging system. Our data show that these two antibodies can be readily assessed simultaneously in mouse xenograft model. This will help guide design of dosing strategies for multiple antibodies and identify potential interaction that could affect pharmacokinetics and possible side effects.

【 授权许可】

Unknown