Durable endothelium-mimicking coating for surface bioengineering cardiovascular stents

Bioactive Materials

Zhilu Yang¹  Yuanyuan Cui²  Nan Huang³  Qiufen Tu⁴  Xiuying Shi⁵  Li Shen⁵  Qing Ma⁵  Manfred F. Maitz⁵  Xing Tan⁵  Kaiqin Xiong⁵  Rui Wang⁶  Zhangmei Hu⁷ 

[1] Corresponding author.;Max Bergmann Center of Biomaterials, Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, 01069, Dresden, Germany;Testing Center, Southwest Jiaotong University, Chengdu, 610031, China;;Analysis &Key Lab of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Yibin Institute of Southwest Jiaotong University, Southwest Jiaotong University, Chengdu, 610031, China;Shanghai Institute of Cardiovascular Diseases, Department of Cardiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China;Shimazu China Co. LTD., No. 180 Yizhou Road, Xuhui District, Shanghai, 200233, China;

关键词: Cardiovascular stents;    Nitric oxide;    Glycocalyx component;    Endothelium mimicking;    Surface bioengineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Mimicking the nitric oxide (NO)-release and glycocalyx functions of native vascular endothelium on cardiovascular stent surfaces has been demonstrated to reduce in-stent restenosis (ISR) effectively. However, the practical performance of such an endothelium-mimicking surfaces is strictly limited by the durability of both NO release and bioactivity of the glycocalyx component. Herein, we present a mussel-inspired amine-bearing adhesive coating able to firmly tether the NO-generating species (e.g., Cu-DOTA coordination complex) and glycocalyx-like component (e.g., heparin) to create a durable endothelium-mimicking surface. The stent surface was firstly coated with polydopamine (pDA), followed by a surface chemical cross-link with polyamine (pAM) to form a durable pAMDA coating. Using a stepwise grafting strategy, Cu-DOTA and heparin were covalently grafted on the pAMDA-coated stent based on carbodiimide chemistry. Owing to both the high chemical stability of the pAMDA coating and covalent immobilization manner of the molecules, this proposed strategy could provide 62.4% bioactivity retention ratio of heparin, meanwhile persistently generate NO at physiological level from 5.9 ± 0.3 to 4.8 ± 0.4 × 10−10 mol cm−2 min−1 in 1 month. As a result, the functionalized vascular stent showed long-term endothelium-mimicking physiological effects on inhibition of thrombosis, inflammation, and intimal hyperplasia, enhanced re-endothelialization, and hence efficiently reduced ISR.

【 授权许可】

Unknown