Apple polyphenol induces cell apoptosis, cell cycle arrest at G2/M phase, and mitotic catastrophe in human bladder transitional carcinoma cells

Journal of Functional Foods

Yi-Ming Kuo¹  Shun-Fa Yang²  Wen-Rong Chen³  Yi-Ru Lee⁴  Yu-Lin Kao⁵  Huei-Jane Lee⁵ 

[1] Department of Urology, Chung Shan Medical University Hospital, Taichung, Taiwan;Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan;Department of Urology, Chung Shan Medical University Hospital, Taichung, Taiwan;Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;

关键词: Apple polyphenols;    Bladder cancer;    Apoptosis;    Cell cycle arrest;    Mitotic catastrophe;   

DOI  :  

来源: DOAJ

【 摘 要 】

Bladder cancer is a common human cancer with very high recurrence rate. Apple polyphenols (APs) possess anti-cancer, anti-oxidation, and anti-inflammation properties. The effect of APs in human bladder transitional cell carcinoma (TCC, TSGH-8301 cells) was examined. The results showed AP reduces cell viability and causes cell cycle arrest at G2/M phase involving the decrease of cyclin B1 and cdc2. AP also increases the subG1 ratio in cell cycle, decreases the mitochondrial membrane potential, and alters the expression of apoptosis related proteins, apoptosis inducing factor (AIF), Bcl2, and caspase 3. It can cause the phenomenon of mitotic catastrophe with multi-nucleation and reduction of microtubule molecule. AP markedly reduces tumor size in TSGH-8301 cells-xenografted examination and induced the pattern of cell death as well as in cell system. In conclusion, AP possesses the potential to mediate cell cycle arrest, apoptosis, and mitotic catastrophe in TCC.

【 授权许可】

Unknown