Apigenin promotes apoptosis, inhibits invasion and induces cell cycle arrest of T24 human bladder cancer cells

Cancer Cell International

Liping Xie¹  Jie Qin¹  Xianglai Xu¹  Xin Xu¹  Jian Wu¹  Zhenghui Hu¹  Yiwei Lin¹  Hong Chen¹  Yeqing Mao¹  Yi Zhu¹ 

[1] Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qing Chun Road, Hangzhou, Zhejiang Province, 310003, China

关键词: Invasion;    Apoptosis;    Cell cycle arrest;    Bladder cancer;    Apigenin;   

Others  :  793665
DOI  :  10.1186/1475-2867-13-54

 received in 2013-02-17, accepted in 2013-05-31,  发布年份 2013

【 摘 要 】

Background

Apigenin (4’,5,7-trihydroxyflavone) was recently shown effective in inhibiting several cancers. The aim of this study was to investigate the effect and mechanism of apigenin in the human bladder cancer cell line T24 for the first time.

Methods

T24 cells were treated with varying concentrations and time of apigenin. Cell viability was evaluated by MTT assay. Cell motility and invasiveness were assayed by Matrigel migration and invasion assay. Flow cytometry and western blot analysis were used to detect cell apoptosis, cell cycle and signaling pathway.

Results

The results demonstrated that apigenin suppressed proliferation and inhibited the migration and invasion potential of T24 bladder cancer cells in a dose- and time-dependent manner, which was associated with induced G2/M Phase cell cycle arrest and apoptosis. The mechanism of action is like to involve PI3K/Akt pathway and Bcl-2 family proteins. Apigenin increased caspase-3 activity and PARP cleavage, indicating that apigenin induced apoptosis in a caspase-dependent way.

Conclusions

These findings suggest that apigenin may be an effective way for treating human bladder cancer.

【 授权许可】

   
2013 Zhu et al.; licensee BioMed Central Ltd.

【 图 表 】

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