Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules

Frontiers in Bioengineering and Biotechnology

Vincenzo Lettera¹  Mario Battisti²  Giorgia Imparato²  Fabrizio A. Pennacchio²  Martina Profeta²  Concetta Di Natale²  Rezvan Jamaledin²  Raffaele Vecchione²  Francesco Urciuolo³  Paolo Antonio Netti³  Costantino Casale⁴ 

[1] Biopox Srl, Naples, Italy;Center for Advanced Biomaterials for Health Care (CABHC), Istituto Italiano di Tecnologia, Naples, Italy;Department of Chemical Materials and Industrial Production (DICMAPI), University of Naples Federico II, Naples, Italy;Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, Italy;

关键词: polymer microneedles;    multi-compartmental;    enzyme;    controlled release;    skin model;   

DOI  :  10.3389/fbioe.2019.00296

来源: DOAJ

【 摘 要 】

Transdermal drug delivery represents an appealing alternative to conventional drug administration systems. In fact, due to their high patient compliance, the development of dissolvable and biodegradable polymer microneedles has recently attracted great attention. Although stamp-based procedures guarantee high tip resolution and reproducibility, they have long processing times, low levels of system engineering, are a source of possible contaminants, and thermo-sensitive drugs cannot be used in conjunction with them. In this work, a novel stamp-based microneedle fabrication method is proposed. It provides a rapid room-temperature production of multi-compartmental biodegradable polymeric microneedles for controlled intradermal drug release. Solvent casting was carried out for only a few minutes and produced a short dissolvable tip made of polyvinylpyrrolidone (PVP). The rest of the stamp was then filled with degradable poly(lactide-co-glycolide) (PLGA) microparticles (μPs) quickly compacted with a vapor-assisted plasticization. The outcome was an array of microneedles with tunable release. The ability of the resulting microneedles to indent was assessed using pig cadaver skin. Controlled intradermal delivery was demonstrated by loading both the tip and the body of the microneedles with model therapeutics; POXA1b laccase from Pleurotus ostreatus is a commercial enzyme used for the whitening of skin spots. The action and indentation of the enzyme-loaded microneedle action were assessed in an in vitro skin model and this highlighted their ability to control the kinetic release of the encapsulated compound.

【 授权许可】

Unknown