Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity

International Journal of Molecular Sciences

DmitryA. Gorbachev¹  KonstantinA. Lukyanov¹  DmitryB. Staroverov²  KarenS. Sarkisyan³ 

[1] Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia;Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia;Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia;

关键词: optogenetics;    genetically encoded photosensitizers;    fluorescent proteins;    phototoxicity;    KillerRed;    SuperNova;   

DOI  :  10.3390/ijms21228800

来源: DOAJ

【 摘 要 】

Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.

【 授权许可】

Unknown