Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways

Cells

Pei Zhi Cheryl Chia¹ 

[1] The Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria 3010, Australia; E-Mail

关键词: optical imaging;    super-resolution microscopy;    flow cytometry;    Golgi apparatus;    endosomes;    fluorescent proteins;   

DOI  :  10.3390/cells2010105

来源: mdpi

【 摘 要 】

Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.