Identification of novel and conserved microRNAs in Coffea canephora and Coffea arabica

Genetics and Molecular Biology

Guilherme Loss-Morais¹  Rogério Margis²  Régis L. Corrêa³  Márcio Alves-Ferreira³  Daniela C.R. Ferreira³ 

[1] Laboratório Nacional de Computação Científica;Universidade Federal do Rio Grande do Sul;Universidade Federal do Rio de Janeiro;

关键词: coffee;    microRNA profiling;    illumina sequencing;   

DOI  :  10.1590/S1415-47572014005000020

来源: DOAJ

【 摘 要 】

As microRNAs (miRNAs) are important regulators of many biological processes, a series of small RNAomes from plants have been produced in the last decade. However, miRNA data from several groups of plants are still lacking, including some economically important crops. Here microRNAs from Coffea canephora leaves were profiled and 58 unique sequences belonging to 33 families were found, including two novel microRNAs that have never been described before in plants. Some of the microRNA sequences were also identified in Coffea arabica that, together with C. canephora, correspond to the two major sources of coffee production in the world. The targets of almost all miRNAs were also predicted on coffee expressed sequences. This is the first report of novel miRNAs in the genus Coffea, and also the first in the plant order Gentianales. The data obtained establishes the basis for the understanding of the complex miRNA-target network on those two important crops.

【 授权许可】

Unknown