Regulatory roles of differentially expressed MicroRNAs in metabolic processes in negative Lens-induced myopia Guinea pigs

BMC Genomics

Meihua Ding¹  Zonghong Li²  Xiaoli Song²  Hongsheng Bi³  Dadong Guo³  Yuanyuan Sun⁴  Guoping Li⁴  Jianfeng Wu⁴  Huixia Wei⁴  Wenjun Jiang⁴ 

[1] Eye Institute of Shandong University of Traditional Chinese Medicine;Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine;Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong;Shandong University of Traditional Chinese Medicine;

关键词: Negative lens-induced myopia;    microRNA profiling;    Guinea pig;    Peroxisome proliferator-activated receptor α;    Metabolic pathway;   

DOI  :  10.1186/s12864-020-6447-x

来源: DOAJ

【 摘 要 】

Abstract Background Myopia is one of the most common vision defects worldwide. microRNAs can regulate the target gene expression, influencing the development of diseases. Results To investigate the alterations of microRNA profiling in negative lens-induced myopia (NLIM) guinea pigs and to explore the regulatory role of microRNAs in the occurrence and the development of myopia, we first established the NLIM guinea pig model after induction for 2 weeks. Further, we isolated sclera to purify total messenger RNA (mRNA) in both NLIM and NLIM fellow sclera. Using next generation sequencing technique and bioinformatics analysis, we identified the differentially expressed microRNAs in NLIM guinea pigs, performed the bioinformatics annotation for the differentially expressed microRNAs, and validated the expression of differentially expressed microRNAs. As a result, we successfully established an NLIM model in guinea pigs, identified 27 differentially expressed microRNAs in NLIM guinea pig sclera, including 10 upregulated and 17 downregulated microRNAs. The KEGG annotation showed the main signaling pathways were closely associated with PPAR signaling, pyruvate and propanoate metabolisms, and TGF-beta signaling pathways. Conclusions Our findings indicate that the development of myopia is mainly involved in the disorder of metabolic processes in NLIM guinea pigs. The PPAR signaling, pyruvate and propanoate metabolism pathways may play roles in the development of myopia.

【 授权许可】

Unknown