DArT, SNP, and SSR analyses of genetic diversity in Lolium perenne L. using bulk sampling

BMC Genetics

Ulf Feuerstein¹  Heiko C. Becker²  Torben Asp³  Siyang Liu⁴  Klaus J. Dehmer⁴  Bruno Studer⁵  Wilbert Luesink⁶  Sabine Schulze⁷ 

[1] Deutsche Saatgutveredelung KG;Georg-August-University Göttingen;Institute of Genetics and Biotechnology, Faculty of Science and Technology, Aarhus University;Leibniz Institute of Plant Genetics and Crop Plant Research (IPK);Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich;Norddeutsche Pflanzenzucht Hans-Georg Lembke KG;Saatzucht Steinach GmbH & Co KG;

关键词: Perennial ryegrass;    Genetic diversity;    Genetic resources;    Genetic pools;    Genotyping;    Hybrid breeding;   

DOI  :  10.1186/s12863-017-0589-0

来源: DOAJ

【 摘 要 】

Abstract Background Lolium perenne L. is the most important forage grass species in temperate regions. It is also considered as a sustainable source of biomass for energy production. However, improvement in biomass yield has been limited by comparison with other major crops. More efficient utilisation of genetic resources and improved breeding schemes are required to advance L. perenne breeding. In an attempt to elucidate the extent of genetic diversity in L. perenne, 1384 DArT, 182 SNP and 48 SSR markers were applied to 297 accessions (Set I) contributed by three German breeding companies and the IPK Genebank. Due to the heterogeneous nature of Lolium accessions, bulk samples were used. Apart from germplasm set I, additional set II and set III was used to determine the reproducibility of marker system and judge the feasibility of bulk strategy in this study. Results By assessing different bulk sizes, 24 individuals per sample were shown to be a representative number of plants to discriminate different accessions. Among the 297 accessions, all marker types revealed a high polymorphism rate; 1.99, 2.00 and 8.19 alleles, were obtained per locus on average using DArTs, SNPs and SSRs, respectively. The Jaccard distance for DArT markers ranged from 0.00 to 0.73, the Modified Roger’s distance (MRD) for SNP markers ranged from 0.03 to 0.52, and for SSR markers from 0.26 to 0.76. Gene diversity for dominant DArT and co-dominant SNP and SSR markers was found to be 0.26, 0.32 and 0.45, respectively. DArT markers showed the highest consistency and reproducibility. Conclusion The resulting data were evaluated using a number of different classification methods, but none of the methods showed a clear differentiation into distinct genetic pools. With regard to hybrid breeding, this will possibly impede substantial progress towards increased biomass yields of L. perenne by utilising heterosis.

【 授权许可】

Unknown