In Vitro Study of Licorice on IL-1β-Induced Chondrocytes and In Silico Approach for Osteoarthritis

Pharmaceuticals

YoungJoon Park¹  Akhtar Ali¹  Wonnam Kim¹  Jaeki Chang²  Yeon Cheol Park³  Bonhyuk Goo⁴  Jong-Hyun Lee⁵  Jong-Sik Jin⁶  Dong-Keun Kim⁶  Hyo-Jin An⁷  Kang-Hyun Leem⁸  Jeonghoon Lee⁹  Shin Seong^1,10 

[1] Cnh Center for Cancer Research, Cnh Corporation, Gangnam-gu, Seoul 06154, Korea;Crop Production & Physiology Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Jeollabuk-do, Korea;Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Korea;Department of Acupuncture & Moxibustion, Kyung Hee University Hospital at Gangdong, Gangdong-gu, Seoul 05278, Korea;Department of Natural Medicine, College of Pharmacy, Dongduk Women’s University, Seongbuk-gu, Seoul 02748, Korea;Department of Oriental Medicine Resources, Jeonbuk National University, Iksan 54596, Jeollabuk-do, Korea;Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju 26339, Gangwon-do, Korea;Division of Pharmacology, College of Korean Medicine, Semyung University, Jecheon 27136, Chungcheongbuk-do, Korea;Herbal Crop Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Chungcheongbuk-do, Korea;Soram Korean Medicine Hospital, Gangnam-gu, Seoul 06154, Korea;

关键词: osteoarthritis;    chondrocyte hypertrophy;    licorice;    Wongam;   

DOI  :  10.3390/ph14121337

来源: DOAJ

【 摘 要 】

Osteoarthritis (OA) is a common degenerative joint disorder that affects joint function, mobility, and pain. The release of proinflammatory cytokines stimulates matrix metalloproteinases (MMPs) and aggrecanase production which further induces articular cartilage degradation. Hypertrophy-like changes in chondrocytes are considered to be an important feature of OA pathogenesis. A Glycyrrhiza new variety, Wongam (WG), was developed by the Korea Rural Development Administration to enhance the cultivation and quality of Glycyrrhizae Radix et Rhizoma (licorice). This study examined the regulatory effect of WG against hypertrophy-like changes such as RUNX2, Collagen X, VEGFA, MMP-13 induction, and Collagen II reduction induced by IL-1β in SW1353 human chondrocytes. Additionally, in silico methods were performed to identify active compounds in licorice to target chondrocyte hypertrophy-related proteins. WG showed inhibitory effects against IL-1β-induced chondrocyte hypertrophy by regulating both HDAC4 activation via the PTH1R/PKA/PP2A pathway and the SOX9/β-catenin signaling pathway. In silico analysis demonstrated that 21 active compounds from licorice have binding potential with 11 targets related to chondrocyte hypertrophy. Further molecular docking analysis and in vivo studies elicited four compounds. Based on HPLC, isoliquiritigenin and its precursors were identified and quantified. Taken together, WG is a potential therapeutic agent for chondrocyte hypertrophy-like changes in OA.

【 授权许可】

Unknown