Identification and characterization of a symbiotic alga from soil bryophyte for lipid profiles

Biology Open

Qi Liu¹  Junping Lv¹  Jia Feng¹  Xiujuan Zhang¹  Guihua Wang¹  Yuning Guo¹  Shulian Xie¹ 

[1] School of Life Science, Shanxi University, Taiyuan 030006, China;

关键词: Symbiotic;    Bryophyte;    Chlorococcum sphacosum;    Phylogenetic analysis;    Lipid accumulation;   

DOI  :  10.1242/bio.019992

来源: DOAJ

【 摘 要 】

A symbiotic alga was successfully isolated from the soil moss Entodon obtusatus found in the Guandi Mountains, Shanxi Province, China, and cultivated under axenic conditions. Morphological observations showed that the symbiotic alga was similar to Chlorococcum. Based on phylogenetic analysis of 18S rRNA and rbcL genes and internal transcribed spacer (ITS) regions, Chlorococcum sp. GD was identified as Chlorococcum sphacosum. The three data sets were congruent for those aspects of the topologies that were relatively robust, and differed for those parts of the topologies that were not. This strain was cultured in BG11 medium to test its growth and biodiesel properties. It produced a lipid content of nearly 40%, and achieved biomass concentration of 410 mg l−1 and lipid productivity of 6.76 mg l−1 day−1, with favorable C16:0 (23.10%) and C18:1 (21.62%) fatty acid content. This alga appears to have potential for use in biodiesel production.

【 授权许可】

Unknown