Knockdown of GmD53a confers strigolactones mediated rhizobia interaction and promotes nodulation in soybean

PeerJ

article

Naveed Rehman¹  Fahim Ullah Khan²  Muhammad Imran³  Shahid Ali Rajput⁴  Yiming Li¹  Ihteram Ullah⁵  Rana waseem Akhtar⁴  Muhammad Imran¹  Arwa Abdulkreem AL-Huqail⁶  Ahmad El Askary⁷  Amany Salah Khalifa⁸  Muhammad Tehseen Azhar⁹ 

[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University

[2]Department of Agriculture, Hazara University

[3]Department of Agriculture, South China Agricultural University

[4]Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture

[5]Department of Plant Breeding & Genetics, Gomal University

[6]Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University

[7]Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University

[8]Department of Clinical Pathology and Pharmaceutics, College of Pharmacy, Taif University

[9]Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University

[10]School of Agriculture Sciences, Zhengzhou University

关键词: GmD53a;    Nitrogen fixation;    Rhizobia positive interaction;    Symbiotic;    Biotic factor;    Sustainability;    Food security;    Signaling;    Soybean nodulation;    Strigolactone;   

DOI  :  10.7717/peerj.12815

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundStrigolactones (SLs) play a key role in modulating plant root growth, shoot branching, and plant-symbiont interaction. However, despite their significance, the components of SL biosynthesis and signaling in soybean and their role in soybean-rhizobia interaction is unknown.MethodsIn this study, we identified and functionally characterized the GmD53a from soybean. The GmD53a ORFs were amplified from root cDNA using primers for GmD53a RNA interference. To induce transgenic hairy roots of soybean, electric shock was used to transform pB7WG1WG2 vectors containing GmD53a knockdown and GUS into K599 strains of Agrobacterium rhizogenes. The hairy roots and nodules were collected and examined for root nodules ratio and RNA was extracted after 4 weeks of rhizobia inoculation.ResultsA tissue-specific expression assay showed that GmD53a was differentially expressed in plant parts, predominantly in the stem and nodule. Furthermore, its expression was significantly up-regulated during rhizobia infection and varied with nodule formation. The GmD53a-knockdown chimerical plants were produced to further check its role in soybean nodulation in comparison with control GUS. In knockdown lines, the GmD53a (suppressor of strigolactone MAX2) has a higher number of nodules compared to control lines. Furthermore, the expression levels of several nodulation genes essential for initiation and formation of nodules were altered in GmD53a-knockdown lines.ConclusionThe results revealed that SL biosynthesis and signaling are not conserved but also have close interaction between SL and legume rhizobia.

【 授权许可】

CC BY