FastProNGS: fast preprocessing of next-generation sequencing reads

BMC Bioinformatics

Zhenhe Yan¹  Xiaoshuang Liu¹  Yang Yang¹  Xiaomin Li¹  Guangxin Zhang¹  Chao Wu² 

[1] Megagenomics Corporation;National Renewable Energy Laboratory CO;

关键词: Quality control;    Adapter removing;    NGS;   

DOI  :  10.1186/s12859-019-2936-9

来源: DOAJ

【 摘 要 】

Abstract Background Next-generation sequencing technology is developing rapidly and the vast amount of data that is generated needs to be preprocessed for downstream analyses. However, until now, software that can efficiently make all the quality assessments and filtration of raw data is still lacking. Results We developed FastProNGS to integrate the quality control process with automatic adapter removal. Parallel processing was implemented to speed up the process by allocating multiple threads. Compared with similar up-to-date preprocessing tools, FastProNGS is by far the fastest. Read information before and after filtration can be output in plain-text, JSON, or HTML formats with user-friendly visualization. Conclusions FastProNGS is a rapid, standardized, and user-friendly tool for preprocessing next-generation sequencing data within minutes. It is an all-in-one software that is convenient for bulk data analysis. It is also very flexible and can implement different functions using different user-set parameter combinations.

【 授权许可】

Unknown