Evaluating Antibacterial Efficacy and Biocompatibility of PAN Nanofibers Loaded with Diclofenac Sodium Salt

Polymers

Nadir Hussain¹  Md.Kaiser Haider¹  MuhammadNauman Sarwar¹  Azeem Ullah¹  Motahira Hashmi¹  IckSoo Kim¹  Sana Ullah¹  MuhammadQamar Khan² 

[1] Nano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Tokida 3-15-1, Ueda, Nagano 386-8567, Japan;Nanotechnology Research Lab, Department of Textile & Clothing, Faculty of Engineering & Technology, National Textile University Karachi Campus, Karachi 74900, Pakistan;

关键词: electrospinning;    biocompatibility;    antibacterial;    wound care;    nanofibers;   

DOI  :  10.3390/polym13040510

来源: DOAJ

【 摘 要 】

Side effects of the drugs’ oral administration led us to examine the possibility of using diclofenac sodium (DLF) in a polymeric drug delivery system based on electrospun polyacrylonitrile (PAN) nanofibers, which can be produced cost-effectively and with good applicability for transdermal treatments. The inclusion of DLF in PAN nanofibers increased the nanofiber diameter from ~200 nm to ~500 nm. This increase can be attributed to the increase in the spinning solution viscosity. FTIR spectra confirm the entrapment of the DLF into the PAN nanofibers. X-ray diffraction pattern showed that the inclusion of the DLF in the PAN nanofibers had caused the misalignment in the polymeric chains of the PAN, thus resulting in a decrease of the peak intensity at 2θ = 17^o. The DLF loaded PAN nanofibers were efficient against the gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E.coli), with maximum inhibition zone of 16 ± 0.46 mm for E.coli and 15.5 ± 0.28 mm for S. aureus. Good cell viability ~95% for L929 cells in more extended incubation periods was reported. A gradual release of DLF from the PAN nanofiber was observed and can be attributed to the stability of Pan in PBS medium. Cell adhesion micrographs show that cell-cell interaction is stronger than the cell-material interaction. This type of weak cell interaction with the wound dressing is particularly advantageous, as this will not disturb the wound surface during the nursing of the wound.

【 授权许可】

Unknown