Humic Substances Mitigate the Impact of Tritium on Luminous Marine Bacteria. Involvement of Reactive Oxygen Species

International Journal of Molecular Sciences

DevardI. Stom¹  GennadiiA. Badun²  OlgaV. Kolesnik³  NadezhdaS. Kudryasheva³  TatianaV. Rozhko⁴ 

[1] Biology Department, Irkutsk State University, 664003 Irkutsk, Russia;Department of Chemistry, Moscow State University, 119991 Moscow, Russia;Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 660036 Krasnoyarsk, Russia;Krasnoyarsk State Medical Academy, 660022 Krasnoyarsk, Russia;

关键词: tritium;    humic substances;    luminous marine bacterium;    bioassay;    detoxification;    reactive oxygen species;   

DOI  :  10.3390/ijms21186783

来源: DOAJ

【 摘 要 】

The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism—luminous bacteria—under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the “hormesis” model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated.

【 授权许可】

Unknown