Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis

Journal of Clinical Medicine

Kinya Tsubota¹  Hiroyuki Shimizu¹  Yoshihiko Usui¹  Masaki Asakage¹  Hiroshi Goto¹  Ryo Wakita¹  Naoya Nezu¹  Masahiro Sugimoto² 

[1] Department of Ophthalmology, Tokyo Medical University, Tokyo 160-0023, Japan;Health Promotion and Preemptive Medicine, Research and Development Center for Minimally Invasive Therapies, Institute of Medical Sciences, Tokyo Medical University, Tokyo 160-8402, Japan;

关键词: Behҫet’s disease;    sarcoidosis;    Vogt-Koyanagi-Harada disease;    metabolomics;    liquid chromatography-mass spectrometry;    biomarker;   

DOI  :  10.3390/jcm9123955

来源: DOAJ

【 摘 要 】

The activities of various metabolic pathways can influence the pathogeneses of autoimmune diseases, and intrinsic metabolites can potentially be used to diagnose diseases. However, the metabolomic analysis of patients with uveitis has not yet been conducted. Here, we profiled the serum metabolomes of patients with three major forms of uveitis (Behҫet’s disease (BD), sarcoidosis, and Vogt-Koyanagi-Harada disease (VKH)) to identify potential biomarkers. This study included 19 BD, 20 sarcoidosis, and 15 VKH patients alongside 16 healthy control subjects. The metabolite concentrations in their sera were quantified using liquid chromatography with time-of-flight mass spectrometry. The discriminative abilities of quantified metabolites were evaluated by four comparisons: control vs. three diseases, and each disease vs. the other two diseases (such as sarcoidosis vs. BD + VKH). Among 78 quantified metabolites, 24 kinds of metabolites showed significant differences in these comparisons. Four multiple logistic regression models were developed and validated. The area under the receiver operating characteristic (ROC) curve (AUC) in the model to discriminate disease groups from control was 0.72. The AUC of the other models to discriminate sarcoidosis, BD, and VKH from the other two diseases were 0.84, 0.83, and 0.73, respectively. This study provides potential diagnostic abilities of sarcoidosis, BD, and VKH using routinely available serum samples that can be collected with minimal invasiveness.

【 授权许可】

Unknown