N-Propionylmannosamine stimulates axonal elongation in a murine model of sciatic nerve injury

Neural Regeneration Research

关键词: microtubule;    axon;    kinesin-5;    Eg5;    regeneration;    monastrol;    molecular motor protein;    aging;    neurodegenerative disorders;    telomere shortening;    MSCs;    cellular therapy;    traumatic brain injury;    spinal cord injuries;    dual diagnosis;    diagnosis;    complications;    rehabilitation;    post-concussion syndrome;    brain concussion;    blood brain barrier;    phage display;    peptide library;    nanocarrier;    targeting;    Schwann cells;    neurite outgrowth;    neuromuscular junction (NMJ);    multiple sclerosis;    TGF-β/BMP-7/Smad signaling;    myogenic differentiation;    Trf3;    tumor suppression;    nerve regeneration;    bone marrow mesenchymal stem cells;    cerebral ischemia;    tail vein injection;    middle cerebral artery occlusion;    cell therapy;    neuroprotection;    nerve regeneration;    brain injury;    neuroimaging;    ferumoxytol;    superparamagnetic iron oxide particles;    human adipose-derived stem cells;    middle cerebral artery occlusion;    intracerebral injection;    magnetic resonance imaging;    enhanced susceptibility-weighted angiography image;    modified neurological severity scores;    rats;    Prussian blue staining;    neural regeneration;    neural regeneration;    non-invasive brain stimulation;    transcranial magnetic stimulation;    neurotrophic factor;    brain-derived neurotrophic factor;    neuroplasticity;    hippocampus;    aging;    cognitive function;    nerve regeneration;    curcumin;    neurons;    HIV-1 gp120 V3 loop;    plasticity;    HIV-associated neurocognitive disorders;    output/input curve;    long-term potentiation;    excitatory postsynaptic potential;    paired-pulse facilitation;    Ca 2+;    synaptosome;    NSFC grants;    neural regeneration;    nerve regeneration;    brain injury;    hydrogen sulfide;    cerebral ischemia/reperfusion injury;    P2X 7 receptor;    2;    3;    5-triphenyl-2H-tetrazolium chloride staining;    animal model;    protection;    sodium hydrosulfide;    immunofluorescence;    middle cerebral artery occlusion;    NSFC grant;    neural regeneration;    nerve regeneration;    γ-aminobutyric acid;    glial fibrillary acidic protein;    glutamic acid decarboxylase;    neurotoxicity;    weaning;    organ index;    cerebrum;    cortex;    glutamate;    neural regeneration;    p53 tumor suppressor gene family;    cerebral ischemia/reperfusion;    pyramidal neurons;    CA1 region;    delayed neuronal death;    immunohistochemistry;    western blotting;    neural regeneration;    nerve regeneration;    spinal cord injury;    rapamycin;    Wnt/β-catenin signaling pathway;    apoptosis;    caspase-3;    brain-derived neurotrophic factor;    neuroprotection;    loss of neurons;    NSFC grants;    neural regeneration;    nerve regeneration;    spinal cord injury;    hydrogen-rich saline;    reactive oxygen species;    physiological saline;    oxidative stress;    Basso;    Beattie and Bresnahan score;    malondialdehyde;    superoxide dismutase;    calcitonin gene-related peptide;    caspase-3;    neural regeneration;    nerve regeneration;    peripheral nerve injury;    rabbits;    sciatic nerve injury;    autologous nerve repair;    polylactic glycolic acid conduit;    extracellular matrix gel;    grafting;    stress relaxation;    creep;    viscoelasticity;    histomorphology;    electrophysiology;    neural regeneration;    nerve regeneration;    peripheral nerve injury;    pain sense model;    dorsal root ganglion;    primary sensory neuron;    glycosylated membrane protein;    sialic acid;    cell electrophoresis;    electrophoresis velocity;    heat-hyperalgesia behavior;    hyperalgesia;    neuraminidase;    neural regeneration;    N-propionylmannosamine;    sialic acid;    glycoengineering;    sciatic nerve;    peripheral nerve;    branching;    arborisation;    Thy1-YFP mouse;    nerve regeneration;   

DOI  :  10.4103/1673-5374.150744

来源: DOAJ

【 摘 要 】

Increasing evidence indicates that sialic acid plays an important role during nerve regeneration. Sialic acids can be modified in vitro as well as in vivo using metabolic oligosaccharide engineering of the N-acyl side chain. N-Propionylmannosamine (ManNProp) increases neurite outgrowth and accelerates the reestablishment of functional synapses in vitro. We investigated the influence of systemic ManNProp application using a specific in vivo mouse model. Using mice expressing axonal fluorescent proteins, we quantified the extension of regenerating axons, the number of regenerating axons, the number of arborising axons and the number of branches per axon 5 days after injury. Sciatic nerves from non-expressing mice were grafted into those expressing yellow fluorescent protein. We began a twice-daily intraperitoneal application of either peracetylated ManNProp (200 mg/kg) or saline solution 5 days before injury, and continued it until nerve harvest (5 days after transection). ManNProp significantly increased the mean distance of axonal regeneration (2.49 mm vs. 1.53 mm; P < 0.005) and the number of arborizing axons (21% vs. 16% P = 0.008) 5 days after sciatic nerve grafting. ManNProp did not affect the number of regenerating axons or the number of branches per arborizing axon. The biochemical glycoengineering of the N-acyl side chain of sialic acid might be a promising approach for improving peripheral nerve regeneration.

【 授权许可】

Unknown