In vivo tracking of human adipose-derived stem cells labeled with ferumoxytol in rats with middle cerebral artery occlusion by magnetic resonance imaging

Neural Regeneration Research

关键词: microtubule;    axon;    kinesin-5;    Eg5;    regeneration;    monastrol;    molecular motor protein;    aging;    neurodegenerative disorders;    telomere shortening;    MSCs;    cellular therapy;    traumatic brain injury;    spinal cord injuries;    dual diagnosis;    diagnosis;    complications;    rehabilitation;    post-concussion syndrome;    brain concussion;    blood brain barrier;    phage display;    peptide library;    nanocarrier;    targeting;    Schwann cells;    neurite outgrowth;    neuromuscular junction (NMJ);    multiple sclerosis;    TGF-β/BMP-7/Smad signaling;    myogenic differentiation;    Trf3;    tumor suppression;    nerve regeneration;    bone marrow mesenchymal stem cells;    cerebral ischemia;    tail vein injection;    middle cerebral artery occlusion;    cell therapy;    neuroprotection;    nerve regeneration;    brain injury;    neuroimaging;    ferumoxytol;    superparamagnetic iron oxide particles;    human adipose-derived stem cells;    middle cerebral artery occlusion;    intracerebral injection;    magnetic resonance imaging;    enhanced susceptibility-weighted angiography image;    modified neurological severity scores;    rats;    Prussian blue staining;    neural regeneration;   

DOI  :  10.4103/1673-5374.158355

来源: DOAJ

【 摘 要 】

Ferumoxytol, an iron replacement product, is a new type of superparamagnetic iron oxide approved by the US Food and Drug Administration. Herein, we assessed the feasibility of tracking transplanted human adipose-derived stem cells labeled with ferumoxytol in middle cerebral artery occlusion-injured rats by 3.0 T MRI in vivo. 1 × 10 4 human adipose-derived stem cells labeled with ferumoxytol-heparin-protamine were transplanted into the brains of rats with middle cerebral artery occlusion. Neurologic impairment was scored at 1, 7, 14, and 28 days after transplantation. T2-weighted imaging and enhanced susceptibility-weighted angiography were used to observe transplanted cells. Results of imaging tests were compared with results of Prussian blue staining. The modified neurologic impairment scores were significantly lower in rats transplanted with cells at all time points except 1 day post-transplantation compared with rats without transplantation. Regions with hypointense signals on T2-weighted and enhanced susceptibility-weighted angiography images corresponded with areas stained by Prussian blue, suggesting the presence of superparamagnetic iron oxide particles within the engrafted cells. Enhanced susceptibility-weighted angiography image exhibited better sensitivity and contrast in tracing ferumoxytol-heparin-protamine-labeled human adipose-derived stem cells compared with T2-weighted imaging in routine MRI.

【 授权许可】

Unknown