Claudin-19 Expression at the Neuromuscular Junction.

axon;claudin-19;nerve terminal;tight junction;neuromuscular junction;Terminal Schwann cells

Okuwa, Sumie ; Jane L. Lubischer, Committee Chair,Robert M. Grossfeld, Committee Member,Brenda J. Grubb, Committee Member,Okuwa, Sumie ; Jane L. Lubischer ; Committee Chair ; Robert M. Grossfeld ; Committee Member ; Brenda J. Grubb ; Committee Member

University:North Carolina State University

关键词: axon;    claudin-19;    nerve terminal;    tight junction;    neuromuscular junction;    Terminal Schwann cells;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/309/etd.pdf?sequence=1&isAllowed=y

美国|英语

来源: null

【 摘 要 】

Terminal Schwann cells (TSCs) sit close together to form a cap over each neuromuscular junction.It is not known how TSCs interact with each other, but their close physical relationship suggests that they might form cell-cell adhesions.Adherens junctions and tight junctions are well-studied formations of cell-cell adhesions and are typically expressed near each other in the same cells.Previous research has shown that M-cadherin, one of the adherens junction proteins, colocalizes with a TSC marker, suggesting the presence ofadherens junctions and tight junctions in TSCs.Additionally, claudin-19, one of the tight junction proteins, is known to be expressed by myelinating Schwann cells, but it is unknown whether claudin-19 is also expressed by TSCs.I therefore hypothesize that claudin-19 is expressed by TSCs and is involved in TSC-TSC interactions.To begin to address this hypothesis, immunolabeling against claudin-19, Schwann cells, and acetylcholine receptors was performed on both cryostat sections and whole-mount of mouse muscles.Claudin-19 expression was found at neuromuscular junctions and was localized to the axon-terminal transition, where myelin ends and TSCs begin.After denervation of the muscle and degeneration of all axons, claudin-19 expression was still present at some neuromuscular junctions.These results show for the first time the expression of claudin-19 in Schwann cells at the rodent neuromuscular junction.