Very low concentration of lipopolysaccharide can induce the production of various cytokines and chemokines in human primary monocytes

BMC Research Notes

Watchara Kasinrerk¹  Ratthakorn Chaiwut¹ 

[1] Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University;

关键词: Chemokine;    Innate immunity;    Lipopolysaccharide;    Monocyte;    Proinflammatory cytokine;   

DOI  :  10.1186/s13104-022-05941-4

来源: DOAJ

【 摘 要 】

Abstract Objective Lipopolysaccharide (LPS), a component of gram-negative bacteria, is a potent innate immune stimulus. The interaction of LPS with innate immune cells induces the production of proinflammatory cytokines and chemokines, thereby leading to the control of infection. In the present study, we investigated the effect of a wide range of LPS concentrations on the regulation of various proinflammatory cytokines and chemokines in human primary monocytes and T lymphocytes. Results We demonstrated that a very low concentration of LPS could regulate the production of cytokines and chemokines in monocytes but not T lymphocytes. Unexpectedly, very low concentrations of LPS (0.0025 and 0.005 ng/mL) could induce TNF-α and IL-6 production, respectively, in monocytes. Our findings provide evidence that in the presence of monocytes, even very low endotoxin contamination could induce cytokine production. We suggest that the recombinant proteins used to investigate immune functions must be thoroughly screened for endotoxins using a highly sensitive method.

【 授权许可】

Unknown